The Active Peptide from Shark Liver (APSL) was expressed in E. coli BL21 cells. The cDNA encoding APSL protein was obtained from shark regenerated hepatic tissue by RT-PCR. then it was cloned in the pET-28a expression vector. https://www.bekindtopets.com/best-grab-Christmas-Let-s-Get-Crackin-Unisex-NuBlend-Crew-Sweatshirt-hot-buy/